PNGase F from Elizabethkingia meningoseptica
Product #：
S0768
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SKU-Pack Size
Stock
Price($)
Quantity
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S0768-50UN
In-stock
457.6
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S0768-300UN
In-stock
2280.2
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Details
l General Information |
Product Name | PNGase F from Elizabethkingia meningoseptica |
Synonym | N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum, Peptide N-glycosidase |
Assay | recombinant, expressed in E. coli, ≥1,000 units/mg protein | CAS Number | 83534-39-8 |
Molecular Weight | mol wt ~36 kDa | MDL number | MFCD00131222 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | lyophilized powder |
l Biological Information |
Biochem/Physiol Actions | Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. |
Application | Highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-teminal 6x histidine fusion tag.Used to deglycosylate protein. |
Unit Definition | One unit will catalyze the release of N-ed oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One unit of PNGase F activity is equal to 1 IUB milliunit. |
l Storage |
Storage temp. | -20°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
