Product Name

Urease from Canavalia ensiformis (Jack bean)

General deion

Subunit molecular weight: ~90,770 ; Composed of six subunits with total molecular weight: ~544,620

Assay

Type III, 500-800 units/mL

CAS Number

9002-13-5

MDL number

MFCD00070858

Suitability

BioReagent

l Physical and Chemical Information

Appearance

glycerol solution

l Biological Information

Biochem/Physiol Actions

Urease activity has been shown to be inhibited by benzpyrene following exposure to light. This inhibition was due to the effect of benzpyrene on urease′s sulfhydryl groups.

Preparation Note

The enzyme is supplied as a glycerol solution. Prepare appropriate dilutions in reaction buffer. The following buffers have been shown not to inhibit urease activity: MES, HEPES, and CHES.

Unit Definition

One micromolar unit will liberate 1.0 μmole of NH₃ from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov