Product Name

Urease from Canavalia ensiformis (Jack bean)

General deion

Subunit molecular weight: ~90,770 ; Composed of six subunits with total molecular weight: ~544,620

Assay

Type C-3, ≥600,000 units/g solid

CAS Number

9002-13-5

MDL number

MFCD00070858

Suitability

BioReagent

l Physical and Chemical Information

Appearance

powder

l Biological Information

Biochem/Physiol Actions

Urease was shown to induce vacuolation in Hela cells, an effect dependent on the ability of urease to convert urea to ammonia.

Preparation Note

The enzyme is soluble in 0.2 M sodium phosphate buffer, pH 7.0, (1 mg/ml) yielding a colorless to light yellow solution with a haze. The following buffers have been shown not to inhibit urease activity: MES, HEPES, and CHES.

Unit Definition

One micromolar unit will liberate 1.0 μmole of NH³ from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov