Product Name

Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)

Synonym

3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase

Assay

≥100 units/mg protein; Protein, ≥50% biuret

CAS Number

37289-07-9

MDL number

MFCD00130789

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Faint Yellow to Faint Brown lyophilized powder

l Biological Information

Biochem/Physiol Actions

The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg₂₊, Cu₂₊, and Cd₂₊) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.

Application

The enzyme has been used in the analysis of bile samples in various studies.

Preparation Note

Partially purified lyophilized powder containing buffer salts and stabilizer

Unit Definition

One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov