Product Name

Penicillin Amidase from Escherichia coli

General deion

Penicillin amidase is a periplasmic 80K heterodimer with A and B chains (209 and 566 amino acids, respectively). It is widely distributed among microorganisms, including bacteria, yeast and filamentous fungi. Among all the sources, the enzyme produced by E. coli is most well-characterized and common for industrial application.

Synonym

Penicillin Acylase, Penicillin Amidohydrolase

Assay

5-10 units/mg protein

CAS Number

9014-06-6

Molecular Weight

Mr ~70000

MDL number

MFCD00147869

Suitability

BioReagent

l Biological Information

Biochem/Physiol Actions

The biosynthesis of Penicillin amidase in E. coli by hydrophobic protein chromatography is an inducible reaction which is regulated by metabolized carbon source (e.g. polyols, carboxylic acid etc.). It is also influenced by catabolite repression.It catalyzes the formation of amide bonds through an acyl-enzyme intermediate.

Application

Penicillin amidase was used to study its effect in release of fatty acid and HSL (homoserine lactone) from AHLs (N -acylhomoserine lactones) in degradation of antibiotics.It was used as positive control for assaying penicillin G acylase activity in the study of functional analysis of bile salt hydrolase and penicillin acylase family members in Lactobacillus sp.Penicillin amidase may be used for synthesis of 6-aminopenicillanic acid from penicillin-G and for the industrial production of β-lactam antibiotics.

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol benzylpenicillin per minute at pH 7.6 and 37°C

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov