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Asparaginase from Escherichia coli

Product #: S0750
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Details

l General Information

Product Name

Asparaginase from Escherichia coli

Synonym

L-Asparagine Amidohydrolase

Assay

100-300 units/mg protein (biuret); Protein, ≥60%

MDL number

MFCD00130565

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off-White Lyophilized powder

l Biological Information

Biochem/Physiol Actions

Asparaginase may cause cell death in leukemic cells by converting the necessary L-asparagine to aspartic acid and ammonia . Asparaginase is allosterically regulated and is crucial for proper cell functioning .

Application

Asparaginase is used in enzymatic assays and to convert asparagine to aspartic acid. Asparaginase is used to reduce the formation of acrylamide in starchy food products. It is also used as a chemotherapy agent for acute lymphoblastic leukemia. The product is from Escherichia coli and is provided as a lyophilized powder containing sodium chloride.

Preparation Note

Lyophilized powder containing sodium chloride

Unit Definition

One unit will liberate 1.0 μmole of ammonia from L-asparagine per min at pH 8.6 at 37 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov

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