Product Name

Papain Agarose from papaya latex

Assay

90-150 units/mL packed gel

Molecular Weight

mol wt 21 kDa

MDL number

MFCD00131791

Suitability

BioReagent

l Physical and Chemical Information

Physical form

Lyophilized powder, stabilized with lactose

l Biological Information

Biochem/Physiol Actions

A cysteine protease that cleaves peptide bonds of basic amino acids, leucine or glycine. pH optimum 6.0-7.0

Also hydrolyzes esters and amides.

Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.

. The pH optimum is 6.0-7.0

. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.

Application

Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P4406 is agarose-ed papain and is provided as a lyophilized powder stabilized with lactose. Product P4406 has been used to digest purified anti-JMJD6 hamster monoclonal antibody into Fab fragments.

Unit Definition

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 6.2 at 25°C, unless otherwise indicated below.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov