Phone:| Tech Support:| E-mail:
Home > Products > Life sciences > Enzymes and proteins > Alpha-Lytic Protease M190A Mutant

Alpha-Lytic Protease M190A Mutant

Product #: S0739
Image SKU-Pack Size Stock Price($) Quantity

Documents

Details

l General Information

Product Name

Alpha-Lytic Protease M190A Mutant

General deion

Alpha-lytic protease (aLP) is an alternative specificity protease for proteomics applications, whose wild-type (WT) version cleaves after T, A, S, and V residues. The M190A (Met190 → Ala190) mutant of aLP has different cleavage specificities, and cleaves after M, F, and L residues. Both the WT and M190A forms of aLP generate peptides of similar average length as trypsin. In WT aLP, the methionines at positions 190 and 213 cause WT aTP to have its particular specificity toward peptide substrates with small hydrophobic side chains at the P1 position. The M190A mutation gives the resulting mutant M190A aLP the ability to cleave peptide substrates with large hydrophobic side chains at the P1 side chain with greater efficiency compared to WT aLP, in addition to accommodating peptides with small hydrophobic side chains as before. Other structural changes in M190A aLP compared to WT aLP have been discussed.

The activity of M190A aLP in the presence of various solution components is as follows:

• 0.1% sodium deoxycholate: ~1.4-fold enhanced activity

• 1.0% sodium deoxycholate: nearly full activity

• 0.1% SDS: ~40% activity

• 1.0% SDS: ~30% activity

• 1 M guanidine HCl: ~20% activity

• 4 M guanidine HCl: ~1% activity (essentially inactivated)

Synonym

ALP M190A, Alpha-Lytic Protease M190A Mutant

Assay

≥0.05 U/mg

Suitability

BioReagent

l Biological Information

Unit Definition

mmols of pNA produced per min per mg protein from 0.5 mM N-succinyl-Ala-Ala-Pro-Phe-PNA at 25 °C in 100 mM Tris-HCl (pH 7.5).

l Storage

Storage temp.

−70°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov

天问科技