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Lysostaphin from Staphylococcus staphylolyticus

Product #: S0732
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l General Information

Product Name

Lysostaphin from Staphylococcus staphylolyticus

General deion

Package size based on protein content

Synonym

Glycyl-glycine Endopeptidase

Assay

≥97% (SDS-PAGE), Protein 40-60 % by biuret, ≥2,000 units/mg protein

CAS Number

9011-93-2

MDL number

MFCD00131556

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off-white / Yellow Cast Yellow Lyophilized Powder

Physical form

Contains potassium phosphate buffer salts and sodium chloride

Solubility(25°C)

Colorless; Clear to Slightly Hazy; 10.00 - 10.50 mg/ml water

l Biological Information

Biochem/Physiol Actions

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-s in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.

pH Optimum for activity: ~7.5

Preparation Note

Contains potassium phosphate buffer salts and sodium chloride

Unit Definition

One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37 °C in a 6.0 ml reaction mixture.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov

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