Thermolysin from Geobacillus stearothermophilus
Product #:
S0703
Image
SKU-Pack Size
Stock
Price($)
Quantity
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S0703-25MG
In-stock
123.2
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+
S0703-100MG
In-stock
322.4
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S0703-250MG
In-stock
634.4
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+
Details
l General Information |
Product Name | Thermolysin from Geobacillus stearothermophilus |
Synonym | Protease from Geobacillus stearothermophilus, Thermophilic-bacterial protease |
Assay | 30-175 units/mg protein (E1%/280) |
CAS Number | 9073-78-3 | MDL number | MFCD00165549 |
Suitability | BioReagent, suitable for cell culture |
l Physical and Chemical Information |
Appearance | White to Yellow to Beige powder |
Physical form | lyophilized powder containing calcium and sodium acetate buffer salts |
l Biological Information |
Application | A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes. |
Preparation Note | lyophilized powder containing calcium and sodium acetate buffer salts. This product is soluble in acetate buffer, pH 7.5 (0.2 mg/ml), yielding a clear to very slightly hazy, colorless solution. |
Unit Definition | One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent). |
l Storage |
Storage temp. | -20°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |