l General Information |
Product Name | HIV Protease Mutant |
Synonym | (L33F, M46L, I54V, V82A, L90M, L10I, M36I, A71V, L63P) |
Assay | recombinant, expressed in E. coli |
Molecular Weight | mol wt ~10.8 kDa |
Suitability | BioReagent |
l Physical and Chemical Information |
Physical form | Supplied as a solution in 0.5M Sodium Acetate, pH 4.7with 50mM Tris-HCL, 1M NaCL, 1mM EDTA, 5mM DTT and 15% glycerol. |
l Biological Information |
Preparation Note | Supplied as a solution in 0.5M Sodium Acetate, pH 4.7with 50mM Tris-HCL, 1M NaCL, 1mM EDTA, 5mM DTT and 15% glycerol. Bioengineered to resist autoproteolysis, resulting in an enzyme with improved stability and substantially higher activity levels over extended time periods to conduct kinetic assays. The enzyme includes the most commonly occurring background polymorphism (L63P) that aids in the drug resistance profile. |
Unit Definition | One unit of HIV protease will cleave 1picomole per minute of the substrate Abz-Ala-Arg-Val-Nle-Tyr(NO2)-Glu-Ala-Nle-NH2 at 37°C. |
l Storage |
Storage temp. | −70°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
