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Home > Products > Life sciences > Enzymes and proteins > Pepsin from porcine gastric mucosa

Pepsin from porcine gastric mucosa

Product #: S0696
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Details

l General Information

Product Name

Pepsin from porcine gastric mucosa

Synonym

Pepsin A, Pepsin from hog stomach

Assay

≥400 units/mg protein

CAS Number

9001-75-6

Molecular Weight

mol wt 35 kDa

MDL number

MFCD00081840

Suitability

BioReagent

l Physical and Chemical Information

Appearance

powder

Solubility(25°C)

10 mM HCl: soluble 1.0 mg/mL, clear to faintly turbid, colorless

l Biological Information

Biochem/Physiol Actions

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin . It does not cleave at valine, alanine, or glycine ages. Z-L-tyrosyl-L-phenylalanine, Z-L-glutamyl-L-tyrosine, or Z-L-methionyl-L-tyrosine may be used as substrates for pepsin digestion. Pepsin is inhibited by several phenylalanine-containing peptides.

Pepsin hydrolyzes peptide bonds, not amide or ester ages. Pepsin cleaves peptides with an aromatic acid on either side of the peptide bond. Sulfur-containing amino acids increase susceptibility to hydrolysis when they are close to the peptide bond. Pepsin preferentially cleaves at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid residues. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide ages.

Application

Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies.

Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice.The product is provided in powder form. The product has been used to denature DNA from kidney cells and to digest pathology samples from anal canal carcinomas (ACC) biopsies prior to EGFR staining.

The enzyme has been used to obtain total vitamin B12 content in food products prior using immunoaffinity columns It has also been used to digest minced soft tissue of snails prior to the isolation of the third stage larvae (L3).

Unit Definition

One unit will produce a ΔA280 of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov

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