Product Name

Pepsin−Agarose from porcine gastric mucosa

General deion

Pepsin is an enzyme found in gastric secretion.

Synonym

Pepsin A

Assay

≥30 units/mg dry solid

Molecular Weight

mol wt 35 kDa

MDL number

MFCD00081840

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Off White to Light Yellow to Light Brown lyophilized powder

Physical form

Lyophilized powder stabilized with lactose

l Biological Information

Biochem/Physiol Actions

Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at Val, Ala or Gly. pH optimum 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH > 6.

Application

Pepsin immobilized onto agarose beads can be used to digest and then separate out the active peptides of the glycoprotein gliadin.

Pepsin-agarose has been used as an immobilization tool to investigate the refolding ability of the pepsin enzyme. It has also been used for the preparation of F(ab′)₂ fragments from rabbit IgG.

Used to produce F(ab′)² fragments of antibodies.

Preparation Note

Lyophilized powder stabilized with lactose

Unit Definition

One unit will produce a ΔA²⁸⁰ of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov