Product Name

Bromelain from pineapple stem

Assay

5-15 units/mg protein; Protein, 30-50% biuret

CAS Number

37189-34-7

MDL number

MFCD00081423

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to White w/ Yellow Cast lyophilized powder

Solubility(25°C)

H²O: soluble 1.0 mg/mL, clear, colorless

l Biological Information

Biochem/Physiol Actions

Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. The product (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.

Application

Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen .

Preparation Note

Lyophilized powder containing mannitol and potassium phosphate buffer salts; Chromatographically purified. Dissolves in water at 1 mg/mL concentration to form a clear, colorless solution.

Unit Definition

One unit will release 1.0 μmole of p-nitrophenol from N-α-Z-L-lysine p-nitrophenyl ester per min at pH 4.6 at 25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov