Product Name

Ficin from fig tree latex

General deion

Extinction Coefficient: E_1% = 21.0 (280 nm); pI: 9.0

Assay

≥1.0 units/mg protein; Protein, 80-100% biuret

CAS Number

9001-33-6

Molecular Weight

mol wt 23.8 kDa

MDL number

MFCD00081597

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Light Brown lyophilized powder

Physical form

Lyophilized powder containing L-cysteine and trace EDTA

l Biological Information

Biochem/Physiol Actions

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported K_m for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)₂ fragments from mouse IgG1.

Preparation Note

Lyophilized powder containing L-cysteine and trace EDTA; This enzyme is soluble in water (10 mg/ml), yielding a clear solution.

Unit Definition

One unit will produce a ΔA²⁸⁰ of 1.0 per min at pH 7.0 at 37°C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path).

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov