l General Information |
Product Name | Papain from papaya latex |
Synonym | Papainase |
Assay | 2× Crystallized, ≥16 units/mg protein | CAS Number | 9001-73-4 |
Molecular Weight | mol wt 21 kDa | MDL number | MFCD00131791 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | White to Light Yellow to Light Brown buffered aqueous suspension |
Physical form | Suspension in 0.05 M sodium acetate, pH 4.5, containing 0.01% thymol |
l Biological Information |
Biochem/Physiol Actions | A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine. pH optimum 6.0-7.0 Also hydrolyzes esters and amides. Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine. . The pH optimum is 6.0-7.0 . Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme. |
Application | Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. |
Preparation Note | Suspension in 0.05 M sodium acetate, pH 4.5, containing 0.01% thymol. Immediately prior to use, the enzyme is typically diluted in buffer containing ~5 mM L-cysteine. |
l Storage |
Storage temp. | 2-8°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
