Product Name

Cathepsin B from human liver

Synonym

Cathepsin B1

Assay

≥1,500 units/mg protein (E1%/280)

CAS Number

9047-22-7

MDL number

MFCD00130748

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous solution

Physical form

Solution in 50 mM sodium acetate, pH 5.0, with 1 mM EDTA.

l Biological Information

Biochem/Physiol Actions

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Preparation Note

Solution in 50 mM sodium acetate, pH 5.0, with 1 mM EDTA.

Unit Definition

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

l Storage

Storage temp.

−70°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov