Product Name

Cathepsin B from bovine spleen

Synonym

Cathepsin B1

Assay

≥10 units/mg protein; Protein, ≥30% biuret

CAS Number

9047-22-7

MDL number

MFCD02662345

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Faint Yellow lyophilized powder

Physical form

Lyophilized powder containing sodium phosphate, sodium chloride and ~6% EDTA as stabilizer.

l Biological Information

Biochem/Physiol Actions

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Preparation Note

Lyophilized powder containing sodium phosphate, sodium chloride and ~6% EDTA as stabilizer.

Unit Definition

One unit will hydrolyze 1 μmole of Z-lysine p-nitrophenyl ester per min at pH 5.0 at 25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov