Product Name

Cathepsin B from human placenta

Synonym

Cathepsin B1

Assay

≥5 units/mg protein; Protein, ~50% Lowry

CAS Number

9047-22-7

MDL number

MFCD00130748

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

Lyophilized powder containing phosphate buffer salts

Solubility(25°C)

H²O: soluble 1.0 mg/mL, clear

l Biological Information

Biochem/Physiol Actions

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Cathepsin B preferentially cleaves at the caboxyl side of Arg-Arg bonds in small molecule substrates. Cathepsin B has been shown to degrade β-amyloid precursor protein into harmless fragments . Cathepsin B inhibition has been shown to limit bone metastasis in breast cancer .

Application

Cathepsin B is a lysosomal cysteine proteinase which hydrolyzes proteins with a broad specificity for peptide bonds. Cathepsin B may be a useful tool in Alzheimer’s research, as it may have a role in the natural defense against the disease . Cathepsin B may be used to cleave procaspase 1 and procaspase 11, and to induce apoptosis in digitonin-permeabilized cells.

Preparation Note

Dissolves in water at 1 mg/mL concentration, yieliding a clear solution. Lyophilized powder containing phosphate buffer salts. This product is soluble in water (1 mg/ml), yielding a clear solution.

Unit Definition

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov