Product Name

Enterokinase from bovine intestine

General deion

Enterokinase is a highly specific serine protease that is used for the removal of the FLAG peptide from N-terminal and Met-N-terminal fusion proteins. It does not remove the C-terminal FLAG.

Synonym

Enteropeptidase

Assay

Suitable for use in flag  - one unit cleaves > 95% of 1 µg N-BAP fusion protein.

CAS Number

9014-74-8

Molecular Weight

mol wt 150 kDa (consisting of 115kDa and 35kDa subunits.)

MDL number

MFCD00131020

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White powder

l Biological Information

Application

Enterokinase is a member of the S1 peptidase family. In vivo, it is responsble for the proteolytic activation of trypsin from trypsinogen. Enterokinase is used for site specific cleavage of recombinant fusion proteins containing an accessible enterokinase recognition site for removal of affinity tags

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov