Product Name

Plasmin from human plasma

General deion

Plasmin functions as a key enzyme of the fibrinolytic cascade, and is also important in inflammation processes.

Synonym

Fibrinolysin

Assay

≥2.0 units/mg protein

CAS Number

9001-90-5

MDL number

MFCD00131921

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

Lyophilized powder containing sodium phosphate, mannitol, and NaCl.

l Biological Information

Biochem/Physiol Actions

Plasmin exhibits preferential cleavage at the carboxyl side of lysine and arginine residues with higher selectivity than trypsin. Converts polymerized fibrin into soluble products.

pH Optimum: 8.5

pH 7.5: ~40% of maximal activity, pH 9.5: ~50% of maximal activity

Temperature Optimum: 37 °C with rapid inactivation at 56 °C

Plasmin is inhibited by α 2-antiplasmin which binds to plasmin blocking its interaction with fibrin. Plasmin digests the N-terminal region of the cytoplasmic protein αsynuclein preventing its uptake by surrounding cells.

Application

A complex between plasmin and an inhibitor has been isolated in a study via affinity chromatography from urokinase-activated human plasma. It has also been used in a study to investigate activation of human epithelial sodium channel (ENaC) by plasmin and chymotrypsin.

Preparation Note

Lyophilized powder containing sodium phosphate, mannitol, and NaCl.

Unit Definition

One unit will produce one μmole of p-Nitroanilide from D-Val-Leu-Lys-p-Nitroanilide per minute at pH 7.5 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov