l General Information |
Product Name | Proteinase, bacterial |
Synonym | Alkaline Protease, Protease from Bacillus licheniformis, Proteinase from Bacillus licheniformis, Subtilo peptidase A |
Assay | Type XXIV, 7.0-14.0 units/mg solid |
CAS Number | 9014-01-1 | MDL number | MFCD00132092 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | White to Light Yellow to Light Beige lyophilized powder |
l Biological Information |
Biochem/Physiol Actions | Proteinase catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate . |
Application | The enzyme from Sigma has been used to optimize release of all mitochondrial populations from homogenized ventricular tissue of rat heart. It has also been used in the pre-hybridisation treatment of formalin fixed, paraffin wax-embedded liver specimens for detecting human and viral DNA. This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. Product P8038, also known as Subtilisin Carlsberg, has been used to hydrolyze cardiac cells to study the silencing of cardiac mitochondrial NHE1. |
Unit Definition | One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent). |
l Storage |
Storage temp. | -20°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
