Product Name

Factor X Activated (Xa) from bovine plasma

Synonym

Factor Xa

Assay

<13 mg/ml (UV); ≥50 units/mg protein; ≥100 units/ml

CAS Number

9002-05-5

MDL number

MFCD00163610

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Colorless Clear aqueous glycerol solution

l Biological Information

Biochem/Physiol Actions

Factor Xa catalyzes the hydrolysis of the Arg-Thr and then Arg-Ile bonds in prothrombin to yield active thrombin.

The fairly strict recognition sequence is Ile-Glu (or Asp)-Gly-Arg-↓-X.

It may sometimes cleave at other basic residues, depending on the conformation of the target protein. Factor Xa will not cleave if a proline residue follows the arginine of the recognition sequence.

pH Optimum: 7.6-8.0

Temperature Optimum: 37 °C

Application

Fusion proteins are commonly expressed with a factor Xa cleavable Ile-Glu (or Asp)-Gly-Arg-↓-X sequence. Typically 1 mg of fusion protein can be incubated with 10 μg of factor Xa for 2.5 hours at 37 °C.

Unit Definition

One unit of activated Factor X will liberate 1.0 μmole of p-nitroanilide from N-benzoyl-L-isoleucyl-L-glutamyl-L-glycyl-L-arginine-p-nitroaniline per minute at pH 8.3 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov