Product Name

Achromopeptidase from bacteria

Synonym

ACP, Achromopeptidase

Assay

≥20,000 units/mg solid

MDL number

MFCD00130333

Suitability

BioReagent

l Physical and Chemical Information

Physical form

Partially purified powder containing lactose.

l Biological Information

Biochem/Physiol Actions

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.

Application

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. Achromopeptidase is from bacteria and is a partially purified powder containing lactose.

Preparation Note

Partially purified powder containing lactose.

Unit Definition

One unit will produce a change in A600 of 0.001 per minute per mL at pH 8.0 at 37 °C using a suspension of Micrococcus lysodeikticus as substrate (1 cm light path).

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov