Product Name

Thrombin from bovine plasma

General deion

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Synonym

Factor Iia

Assay

≥2,000 NIH units/mg protein (E1%/280 = 19.5)

CAS Number

9002-04-4

MDL number

MFCD00166053

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

Lyophilized from saline sodium citrate buffer, pH 6.5

l Biological Information

Biochem/Physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Application

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess a novel antiplatelet therapy in acute coronary syndromes.

Preparation Note

Lyophilized from saline sodium citrate buffer, pH 6.5. Traditional thrombin products are activated with bovine brain, whereas Thrombin is activated with bovine lung and does not contain any bovine brain products. The product is soluble in water (10 mg/ml), yielding a clear solution.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov