Product Name

Trypsin Acetylated from bovine pancreas

General deion

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Synonym

Acetyltrypsin

Assay

Type V-S, Protein (Biuret): 80 - 100%; ≥8,500 BAEE units/mg protein (biuret)

MDL number

MFCD00082094

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off-White Powder

l Biological Information

Biochem/Physiol Actions

Lys-acetylated trypsin is less susceptible to autolysis than the native form.

Application

Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter.

Preparation Note

Prepared by the method of Labouesse, J. and Gervais, M., Eur. J. Biochem., 2, 215 (1967).

Unit Definition

One BAEE unit will produce a ΔA²⁵³ of 0.001 per min at pH 7.6 at 25°C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov