Product Name

Elastase from human leukocytes

Synonym

Lysosomal elastase

Assay

≥50 units/mg protein (Bradford)

CAS Number

9004-06-2

MDL number

MFCD00130997

l Physical and Chemical Information

Appearance

White to Off-White lyophilized powder

Physical form

Lyophilized from 0.05 M sodium acetate (pH 5.5) and 0.6 M NaCl

l Biological Information

Biochem/Physiol Actions

Unlike pancreatic elastase the leukocyte enzyme has a preferential cleavage for the carboxyl side of valine, but will also cleave to a lesser extent after alanine. Natural substrates include elastin, cartilage proteoglycans, collagen types I, II, II and IV, and fibronectin.

Application

Elastase has been used to digest fibronectin. The results were compared with fibronectin digestion by crude human leukocyte homogenateto examine the presence of fibronectin peptides in saliva of patients with Sjögren′s syndrome.It has also been used as a reference to determine the elastase activity in cell lysates. This study examined the effect of all-trans retinoic acid on procoagulant and fibrinolytic activities of cultured blast cells. These blast cells were from patients with acute promyelocytic leukemia.

Elastase from human leukocytes has been used in a study that determined that fragments of Nle3-angiotensin(1-7) accelerate healing in dermal models.

Unit Definition

One unit will release one nanomole of p-nitrophenol per sec from BOC-L-alanine p-nitrophenyl ester at pH 6.5 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov