Product Name

Elastase from porcine pancreas

General deion

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.

Synonym

Elastase from hog pancreas, Pancreatopeptidase E

Assay

suitable for cell culture; ≥4 units/mg protein

CAS Number

39445-21-1

MDL number

MFCD00130998

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Yellow to Beige lyophilized powder

l Biological Information

Biochem/Physiol Actions

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester.

Application

Elastase from porcine pancreas is used for the following applications:

• Cell culture techniques

• ELISA techniques

• Used in assay for elastase inhibitory activity

Unit Definition

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov