Pyroglutamate Aminopeptidase from Pyrococcus furiosus
Product #：
S0596
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S0596-0.01UN
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1380.6
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l General Information |
Product Name | Pyroglutamate Aminopeptidase from Pyrococcus furiosus |
Synonym | L-Pyrrolidone carboxyl peptidase |
Assay | recombinant, expressed in E. coli, ~90% (SDS-PAGE), ≥5.0 units/mg protein |
Molecular Weight | mol wt 24.072 kDa by amino acid sequence; mol wt 28 kDa by SDS-PAGE | MDL number | MFCD00132139 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | Lyophilized Powder |
Physical form | Lyophilized powder containing sodium phosphate |
l Biological Information |
Biochem/Physiol Actions | This enzyme is specific for N-terminal pyroglutamic acids. It cleaves the N-terminal pyroglutamic acid from proteins and peptides prior to Edman degradation. The optimal temperature range is 95 to 100 °C and the optimal pH range is 6.0 to 9.0. |
Application | Thermostable aminopeptidase that liberates N-terminal pyroglutamic acid from proteins and peptides prior to Edman degradation. Pyroglutamate Aminopeptidase, from Pyrococcus furiosus is a recombinant, thermostable aminopeptidase that is expressed in Escherichia coli. It is used to cleave pyroglutamic acid which allows analysis of N-terminal sequences of peptides. Thermostable aminopeptidase has been used for the removal of pyroglutamate (pGlu) N-terminal blocking group, under reduced conditions, prior to N-terminal sequencing of purified cassiicolin. |
Preparation Note | Reconstitute the vial of enzyme with 50 μl of 50 mM sodium phosphate, pH 7.0, with 10 mM DTT and 1 mM EDTA. The reconstituted solution should be stored at -20 °C. |
Unit Definition | One unit will hydrolyze 1 μmol of pyroglutamate p-nitroanilide per minute at pH 7.0 at 37 °C. |
l Storage |
Storage temp. | -20°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
