Product Name

Carboxypeptidase G from Pseudomonas sp.

Synonym

γ-Glutamyl hydrolase

Assay

≥3 units/mg protein; Protein, ~70% biuret

CAS Number

9074-87-7

MDL number

MFCD00130719

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Pink, Yellow, Orange or Brown lyophilized powder

Physical form

Contains sodium acetate salt.

Solubility(25°C)

H²O: soluble 0.5 mg/mL

l Biological Information

Biochem/Physiol Actions

Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves γ-glutamyl pteroyl poly-γ-glutamate yielding pteroyl-α-glutamate (folic acid) and free glutamate. It is considered highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid of the leaving group. Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn₂₊ ions.

Application

Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO‑cGMP signaling in lung adenocarcinoma cells.

Preparation Note

Chromatographically purified; Solutions should be prepared fresh prior to use.

Unit Definition

One unit will hydrolyze 1.0 μmole of L-glutamic acid from (+)amethopterin per min at pH 7.3 at 30 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov