Product Name

Carboxypeptidase A−Agarose

Synonym

Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase

Assay

≥6 units/mL packed gel, 25 °C, enzyme from bovine pancreas

Molecular Weight

mol wt ~35,250

MDL number

MFCD00130718

Suitability

BioReagent

l Physical and Chemical Information

Appearance

ammonium sulfate suspension

Physical form

Suspension in 2.0 M (NH⁴)²SO⁴, pH 7

l Biological Information

Biochem/Physiol Actions

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by β-phenylpropionate and indole acetate.

Unit Definition

One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov