Product Name

Prolidase from porcine kidney

Synonym

Aminoacyl-L-proline hydrolase, Imido Dipeptidase, Prolidase, Proline dipeptidase

Assay

≥100 units/mg protein; Protein, 20-60% Lowry

CAS Number

9025-32-5

MDL number

MFCD00132079

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

Supplied as a lyophilized powder containing Tris buffer salt and MnCl².

l Biological Information

Biochem/Physiol Actions

Prolidase is an enzyme that catalyzes the hydrolysis of the imide bond between an α-carboxyl group and proline or hydroxyproline. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues.

Application

Prolidase is has an important role recycling of proline and collagen production. It is used to study mutations in the PEPD gene that cause prolidase deficiency. It is used to hydrolyze proteins with C-terminal proline or hydroxyproline residues. Prolidase, product P6675 from porcine kidney, has been used to hydrolyze peptide bonds from the amino terminus when studying enzymatic methylation of membrane proteins.

Unit Definition

One unit will hydrolyze 1.0 μmole of Gly-Pro per min at pH 8.0 at 40 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov