Synonym

Leucine Aminopeptidase IV

Assay

≥200 units/mg protein; Protein, 40-60% Lowry

CAS Number

9031-94-1

MDL number

MFCD00166325

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Faint Yellow Lyophilized Powder

Physical form

Contains calcium acetate

l Biological Information

Biochem/Physiol Actions

Aminopeptidase I from S. griseus has a fairly broad specificity, being able to remove the N-terminal residue of most proteins, except where the penultimate residue is an imino acid. It contains two Zn₂₊ binding sites. Aminopeptidase I from S. griseus is inhibited by 1,10-phenanthroline and is activated six-fold by Ca₂₊, which also stabilizes it against heat inactivation. This monomeric zinc metalloprotein has an isoelectric point (pI) of 5.4.

Application

Aminopeptidase I from Streptomyces griseus may be used as a reagent for the analysis of protein structure and as a model for studies of proteolytic enzyme activation by calcium ions. It may be used as a reagent in the assay of endoprotease activities with a synthetic substrate in a two-stage assay. The lyophilized powder also contains calcium acetate.

Preparation Note

Reconstitute in 20 mM tricine, pH 8.0, with 0.05% bovine serum albumin. Dilute the enzyme with the reconstitution buffer to 0.15-0.3 U/mL for a working concentration. Solutions should be prepared fresh prior to use.

Unit Definition

One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 8.0, 25 °C and 3.0 mM substrate concentration.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov