Product Name

Aminopeptidase from Aeromonas proteolytica

General deion

A zinc-containing enzyme.

Assay

50-150 units/mg protein; Protein, ~40% biuret

CAS Number

37288-67-8

MDL number

MFCD00166325

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to White w/ Yellow Cast Lyophilized Powder

Physical form

Lyophilized powder containing tricine buffer, pH 8.0, zinc chloride and stabilizer.

Solubility(25°C)

H²O: soluble 0.9-1.1 mg/mL, clear, colorless

l Biological Information

Biochem/Physiol Actions

Aminopeptidase from Aeromonas proteolytica is involved in protein maturation, hormone production and peptide digestion.

Aminopeptidase from Aeromonas proteolytica is a metalloenzyme, which contains 2 atoms of Zn₂₊ in a single polypeptide with an approximate molecular weight of 29.5 kDa as determined by sedimentation. This enzyme has a high degree of stability, being stable even at temperatures of 70 °C for several hours. Partial inactivation occurs in 8 M urea. Maximum stability and activity are between pH 8.0-8.5. Aminopeptidase from Aeromonas proteolytica can function as an esterase.

Application

Aminopeptidases are a family of widely distributed proteases, which may be used to study many significant biological processes such as protein maturation, hormone production, and peptide digestion. The enzyme has been used to measure the kinetic rate constant for the binding of bestatin, a general protease inhibitor, to aminopeptidase.

Preparation Note

Dissolves in water at 0.9-1.1 mg/mL concentration to form a clear, colorless solution.

Unit Definition

One unit will hydrolyze 1.0 μmole of L-leucine p-nitroanilide to L-leucine and p-nitroaniline per min at pH 8.0 at 25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov