Product Name

β-Galactosidase-biotin labeled from Escherichia coli

Assay

350-1200 units/mg protein; Protein, ~75% E1%/280

MDL number

MFCD00130626

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White lyophilized powder

Physical form

Lyophilized powder containing Tris-acetate, DTT, MgCl², and isopropyl β-D-thiogalactopyranoside

l Biological Information

Biochem/Physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Application

β-Galactosidase was used as a control antigen in the selection of human antibody fragments by phage display.

Preparation Note

Biotinylated using biotin–maleimide which incorporates an extended spacer.

Unit Definition

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside per min at pH 7.3 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov