Product Name

Fpg Protein from Escherichia coli

General deion

Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme found in Escherichia coli, which contains one zinc atom. Proximal to its C-terminal, it contains a zinc-finger motif of CC/CC type.

Synonym

Fapy DNA glycosylase, Formamidopyrimidine DNA glycosylase, MutM

Assay

≥90% (SDS-PAGE), recombinant, expressed in E. coli, >20,000 units/mg protein

CAS Number

78783-53-6

Molecular Weight

mol wt 30.2 kDa (269 amino acids, predicted from the nucleotide sequence)

MDL number

MFCD02264758

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Colorless buffered aqueous glycerol solution

Physical form

Solution in 50% glycerol containing 50 mM potassium HEPES, pH 7.5, 1 mM DTT, 1 mM EDTA, and 200 mM NaCl.

l Biological Information

Biochem/Physiol Actions

Formamidopyrimidine-DNA glycosylase (Fpg) cleaves double-stranded DNA containing the damaged base 8-oxo-7,8-dihydroguanine. It functions as an N-glycosylase and apurinic/apyrimidinic lyase.

Application

Fpg Protein from Escherichia coli has been used for the assessment of DNA oxidative damage using comet assay.

Unit Definition

One unit will cleave 50% of 0.5 pmol of double-stranded DNA oligomer substrate (8-oxoguanine−mutated) in 10 min at 25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov