Product Name

O-Glycosidase from Streptococcus pneumoniae

Synonym

Endo-α-N-acetylgalactosaminidase, O-Glycanase

Assay

recombinant, expressed in E. coli, ≥1.25 U/ml

CAS Number

9032-92-2

MDL number

MFCD00131003

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous solution

Physical form

Solution in 50 mM sodium phosphate, pH 7.5

l Biological Information

Biochem/Physiol Actions

Releases unsubstituted Ser- and Thr-ed β-Gal-(1→3)-α-GalNAc (Core 1 type O-glycan) from glycoproteins. Substitutions of the disaccharide core with sialic acid, lactosamine (galactose-N-acetyl glucosamine), or fucose will block hydrolysis and prevent the liberation of the oligosaccharide from the protein. Pretreament with glycolytic enzymes to remove substituent saccharides from the O-glycan may be needed prior to cleavage using O-glycosidase..

Unit Definition

One unit will hydrolyze 1 μmole of p-nitrophenyl galacto-N-bioside (β-Gal-(1→3)-α-GalNAc-1→ΟC⁶H⁴NO²) per min at 37 °C at pH 6.5.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov