β-N-Acetylglucosaminidase from bovine kidney
Product #:
S0550
Image
SKU-Pack Size
Stock
Price($)
Quantity
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S0550-5UN
In-stock
748.8
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S0550-10UN
In-stock
1326
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Details
l General Information |
Product Name | β-N-Acetylglucosaminidase from bovine kidney |
Synonym | β-N-Acetyl-D-hexosaminide N-acetylhexosaminohydrolase, β-N-Acetylhexosaminidase |
Assay | 10-50 units/mg protein |
CAS Number | 9012-33-3 | MDL number | MFCD00081402 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | ammonium sulfate suspension |
Physical form | Suspension in 3.2 M (NH4)2SO4, pH approx. 6.0 |
l Biological Information |
Biochem/Physiol Actions | This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-ed N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β-D-glucosaminide. β-N-actylglucosaminidase hydrolyzes the terminal nonreducing N-acetyl-D-hexosamine residues. This enzyme contains two predominant isozymes, Hex A, a heterodimer, and Hex B, a homodimer. N-acetylglucosamine, acetamide, N-2-acetamido-2-deoyglucosylamine, N-acetylnojirimycin, and N-acetyldeoxynojirmycin are known inhibitors. |
Application | Bovine kidney β-N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides based on their effective transglycosylation of β-GlcNAc and β-GalNAcc. It may be a useful tool to study Alzheimer’s Disease. |
Preparation Note | This product is soluble in water (1 mg/ml) |
Unit Definition | One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at the pH 4.25 at 25 °C. |
l Storage |
Storage temp. | 2-8°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |