Product Name

Cellulase from Trichoderma reesei ATCC 26921

Synonym

1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase

Assay

≥1 unit/mg solid

CAS Number

9012-54-8

MDL number

MFCD00081510

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Solubility(25°C)

deionized water: soluble 5.0 mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB).)

l Biological Information

Biochem/Physiol Actions

Cellulases are enzymes that hydrolyze cellulose to glucose. Cellulase is used to study the development of occupational asthma in the detergent, pharmaceutical, baking, and enzyme production industries. Cellulase is added to detergents to improve cleansing properties.It is also applied in the biodegradation of paper products such as foolscap paper, filter paper, newspaper and office paper as well as microcrystalline cellulose.

Application

Cellulase has been used to degrade cello-oligosaccharides into glucose; to study the biodegradability of bioabsorbable bacterial cellulose (BBC) material.

Cellulase from Trichoderma reesei has been used in a study to assess the differential regulation of the cellulase tranion factors XYR1, ACE2, and ACE. Cellulase from Trichoderma reesei has also been used in a study to investigate the liquefaction of hydrothermally pretreated wheat straw at high-solids content.

Preparation Note

Dissolves in sterile deionized (DI) water in the presence of 0.15% polyhexamethylene biguanide (PHMB) at 5 mg/mL concentration.

Unit Definition

One unit will liberate 1.0 μmole of glucose from cellulose in one hr at pH 5.0 at 37 °C (2 hr incubation time).

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov