Product Name

Cellulase from Aspergillus niger

General deion

The FDA recognizes cellulase from A. niger as GRAS (Generally Regarded As Safe) if non-pathogenic and non-toxigenic strains, as well as current good manufacturing practices, are used in production.

Synonym

1,4-(1,3:1,4)-β-D-Glucan 4-glucanohydrolase

Assay

≥0.3 units/mg solid

CAS Number

9012-54-8

MDL number

MFCD00081510

Suitability

BioReagent

l Physical and Chemical Information

Appearance

powder

l Biological Information

Biochem/Physiol Actions

Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic ages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine age.

Application

Cellulase has been used to study the ability of several of its possible substrates, cellulose, Avicel PH-101, and filter paper, to protect enzyme activity during monogastric diegstion in animal and avian digestive tracts.

The enzyme has also been approved as a secondary direct food additive as an aid in clam and shrimp processing.

Unit Definition

One unit will liberate 1.0 μmole of glucose from cellulose in one hr at pH 5.0 at 37 °C (2 hr incubation time).

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov