Product Name

β-Glucuronidase from Escherichia coli

Synonym

β-D-Glucuronide glucuronosohydrolase

Assay

≥20,000 units/mg protein, recombinant, expressed in E. coli overproducing strain

CAS Number

9001-45-0

MDL number

MFCD00130629

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off White lyophilized powder

Solubility(25°C)

15 mg/ml of 0.075 M Potassium Phosphate, pH 6.8; Colorless; Clear to Slightly Hazy

l Biological Information

Biochem/Physiol Actions

β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Application

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene .

Unit Definition

One modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov