Product Name

β-Glucuronidase from Helix pomatia

General deion

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Synonym

β-D-Glucuronide glucuronosohydrolase

Assay

Type HP-2, ≥100,000 units/mL

CAS Number

9001-45-0

MDL number

MFCD00130629

Suitability

BioReagent

l Physical and Chemical Information

Appearance

aqueous solution

Physical form

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

l Biological Information

Biochem/Physiol Actions

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

β-Glucuronidase from Helix pomatia is a mixture of enzymes derived from the Roman snail.

Optimal pH:

• Glucuronidase activity: 4.5 to 5.0

• Sulfatase activity: ~6.2

Inhibitors: D-glucuronic acid, D-galacturonic acid, D-glucaro-1, 4-lactone

Application

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene .

β-glucuronidase was used in enzymic hydrolysis of tissue homogenates for liquid chromatography-electrospray ion trap mass spectrometry (LC/MSn) analysis, to study the structures of degradation products of baicalin.

Unit Definition

One modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov