Product Name

β-Glucuronidase from Helix aspersa (garden snail)

Assay

Type HA-4; ≥300,000 units/g solid solid; ≤7,500 units/g solid sulfatase (secondary activity)

MDL number

MFCD00130629

Suitability

BioReagent

l Physical and Chemical Information

Appearance

partially purified powder

Solubility(25°C)

H²O: soluble 1.90-2.10 mg/mL, clear to slightly hazy

l Biological Information

Biochem/Physiol Actions

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Application

β-glucuronidase was used in enzymic hydrolysis of tissue homogenates for liquid chromatography-electrospray ion trap mass spectrometry (LC/MSn) analysis, to study the structures of degradation products of baicalin.

Unit Definition

One modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37°C at the pH 5.0 (30 min assay).

One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov