β-Glucuronidase from Helix pomatia
Product #:
S0519
Image
SKU-Pack Size
Stock
Price($)
Quantity
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S0519-100KU
In-stock
199.4
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+
S0519-500KU
In-stock
629.2
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S0519-1MU
In-stock
1097.2
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+
S0519-2MU
In-stock
1892.8
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+
Details
l General Information |
Product Name | β-Glucuronidase from Helix pomatia |
General deion | β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity. |
Synonym | β-D-Glucuronide glucuronosohydrolase |
Assay | Type H-1, partially purified powder, ≥300,000 units/g solid; ≥10,000 units/g solid sulfatase (secondary activity) |
CAS Number | 9001-45-0 | MDL number | MFCD00130629 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | White to Light Yellow to Light Brown Powder |
l Biological Information |
Biochem/Physiol Actions | Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption. β-Glucuronidase from Helix pomatia is a mixture of enzymes derived from the Roman snail. Optimal pH: • Glucuronidase activity: 4.5 to 5.0 • Sulfatase activity: ~6.2 Inhibitors: D-glucuronic acid, D-galacturonic acid, D-glucaro-1, 4-lactone |
Application | β-glucuronidase is used as a reporter gene in GUS assays to monitor gene . |
Unit Definition | One modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay). Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C. |
l Storage |
Storage temp. | -20°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |