Product Name

α-Mannosidase from Canavalia ensiformis (Jack bean)

General deion

αMannosidase from Canavalia ensiformis is a tretamer composed of two subunits that each contain two components at 44 and 66 kDa.

Synonym

α-D-Mannoside mannohydrolase

Assay

≥15 units/mg protein (biuret)

CAS Number

9025-42-7

MDL number

MFCD00130495

Suitability

BioReagent

l Physical and Chemical Information

Appearance

ammonium sulfate suspension

Physical form

Suspension in 3.0 M (NH⁴)²SO⁴ and 0.1 mM zinc acetate, pH 7.5

l Biological Information

Biochem/Physiol Actions

α-Mannosidase is an acid hydrolase which is located in plant vacuoles and is thought to be involved with the turnover of N-ed glycoproteins.

αMannosidase has been shown to inhibit the proliferation of B-lymphocytes.

Application

Liberates mannose from a variety of synthetic and natural α-mannosides.

α-Mannosidase can be used to liberate mannose from a variety of synthetic and natural α-mannosides. It has also been used in a study to investigate the causes of neurodegeneration in mucolipidosis II ′knock-in′ mice.

Preparation Note

This enzyme is soluble in water (1 mg/ml), yielding a clear and colorless solution.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-D-mannoside to p-nitrophenol and D-mannose per min at pH 4.5 at 25°C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov