Product Name

β-Galactosidase from Escherichia coli

Synonym

β-D-Galactoside galactohydrolase, Lactase

CAS Number

9031-11-2

MDL number

MFCD00130623

Molecular Weight

mol wt ~116 kDa

Suitability

BioReagent, for use as a marker in SDS-PAGE

l Physical and Chemical Information

Appearance

White to Off-White Powder

l Biological Information

Biochem/Physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Application

β-Galactosidase from Escherichia coli has been used as an internal standard in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). It has been used in the protein ladder mix for optimization of microfluidic western blotting assay.

Unit Definition

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov