Product Name

β-Galactosidase from Escherichia coli

Synonym

β-D-Galactoside galactohydrolase, Lactase

Assay

≥500 units/mg protein

CAS Number

9031-11-2

MDL number

MFCD00130623

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off-White lyophilized powder

Physical form

Stabilized with phosphate buffer and sucrose

l Biological Information

Biochem/Physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Application

β-Galactosidase may be used for derivatization, such as an enzyme label for IgG, without prior dialysis or gel filtration.

Unit Definition

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactopyranoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov