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β-Galactosidase from Escherichia coli

Product #: S0494
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Details

l General Information

Product Name

β-Galactosidase from Escherichia coli

Synonym

β-D-Galactoside galactohydrolase, Lactase

Assay

for enzyme immunoassay, ~140 U/mg

CAS Number

9031-11-2

Molecular Weight

Mr ~135000

MDL number

MFCD00130623

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Lyophilized powder

l Biological Information

Biochem/Physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Application

Aqueous solution may be used directly for derivatization without previous dialysis or gel permeation chromatography.

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 2-nitrophenyl-β-D-galactopyranoside per minute at pH 7.8 and 37 °C

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov

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