Product Name

α-Galactosidase I, Alkaline from Cucumis melo

General deion

Alkaline α-Galactosidase I is a glycoside hydrolase, separated from the extracts of melon fruits. The activity of this enzyme is found to increase during the early stages of ovary development and fruit set.

Synonym

α-D-Galactoside Galactohydrolase, Melibiase

Assay

≥85% (SDS-PAGE); ≥20 units/mg protein; expressed in E. coli

CAS Number

9025-35-8

Molecular Weight

apparent mol wt ~84 kDa by SDS-PAGE

MDL number

MFCD00130486

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Lyophilized Powder

Physical form

The product is supplied as a lyophilized powder containing Tris-HCl buffer salts, DTT, EDTA, and NaCl.

l Biological Information

Biochem/Physiol Actions

α-Galactosidase form I from melon may play a significant role in photoassimilate partitioning in sink tissue. The form I enzyme has a preferred activity with raffinose and significant activity with stachyose. A unique feature of this enzyme is that it exhibits weak product inhibition by galactose. This enzyme shows a preference for raffinose and also activity with stachyose. Unlike other a-Galctosidases, this enyzme also shows weak inhibition by galactose.

Preparation Note

Reconstitution with 0.9 ml of 0.05% (v/v) TWEENÒ 20 aqueous solution will yield a solution containing ~0.5 mg-protein/ml, 50 mM Tris-HCl, pH 7.5, 1 mM DTT, 0.1 mM EDTA, 200 mM NaCl, and 0.05% TWEEN 20.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-D-galactoside to p-nitrophenol and D-galactose per minute at pH 7.8 at 30 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov