l General Information |
Product Name | β-Amylase from barley |
Synonym | 1,4-α-D-Glucan maltohydrolase |
Assay | Type II-B, Crude, 20-80 units/mg protein (biuret); protein: ≥60% by biuret |
CAS Number | 9000-91-3 | MDL number | MFCD00081391 |
Suitability | BioReagent |
l Biological Information |
Biochem/Physiol Actions | β-Amylase hydrolyzes the α-(1,4) glucan ages in polysaccharides of three or more α-(1,4) ed D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. Pure, crystalline β-amylase preparation consists of four isoenzymes with different isoelectric points. The enzyme polymerizes very rapidly through the sulfhydryl groups in the absence of reducing agents. p-Chloromercuribenzoate inhibits the polymerization and the enzymatic activity. The reducing agents mercaptoethanol or dithiothreitol can completely restore the activity. |
Application | β -Amylase is used to hydrolyze α bonds of α-ed polysaccharides, such as starch and glycogen. β -Amylase, has been used in various plant studies, such as carbon starvation studies in Populus tremuloides . β -Amylase, from barley, has been used to study how pressure and temperature affect catalytic activity . |
Unit Definition | One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 4.8 at 20 °C. |
l Storage |
Storage temp. | 2-8°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
